2×Taq PCR MasterMix
● 產(chǎn)品包裝：
|
|
目錄號(hào)
|
包裝
|
價(jià)格
|
|
2×MasterMix（不含染料）
|
RTC3103-02
|
500μl
|
60元
|
|
RTC3103-03
|
5×500μl
|
240元
|
|
2×MasterMix（含染料）
|
RTC3104-02
|
500μl
|
60元
|
|
RTC3104-03
|
5×500μl
|
240元
|
注：以50μl PCR反應(yīng)體系為例，每次使用25μl 2×MasterMix，500μl可做20次 50μl PCR反應(yīng)。
● 儲(chǔ)存條件: -20℃可長(zhǎng)期保存；4℃穩(wěn)定貯存3個(gè)月。經(jīng)常使用時(shí)，一旦融化后請(qǐng)4℃貯存，盡量避免反復(fù)凍融。
● 產(chǎn)品簡(jiǎn)介：
本產(chǎn)品包含Taq DNA聚合酶、dNTPs、MgCl2、反應(yīng)緩沖液、PCR反應(yīng)的增強(qiáng)劑和優(yōu)化劑以及穩(wěn)定劑，濃度為2×。使用時(shí)，只需補(bǔ)加實(shí)驗(yàn)所需的引物和模板后即可進(jìn)行PCR反應(yīng)，可最大限度的減少人為誤差，具有快速簡(jiǎn)便、穩(wěn)定性好等優(yōu)點(diǎn)。適用于常規(guī)PCR反應(yīng)、復(fù)雜模板（如GC含量高，有二級(jí)結(jié)構(gòu)）的擴(kuò)增以及大規(guī)?；驒z測(cè)。使用該產(chǎn)品得到的PCR擴(kuò)增產(chǎn)物末端含有一個(gè)A堿基，可以直接用于TA克隆。
● 質(zhì)量控制：
經(jīng)檢測(cè)無(wú)外源核酸酶活性；PCR方法檢測(cè)無(wú)宿主殘余基因組DNA；能有效地?cái)U(kuò)增人基因組中的單拷貝基因。
● 使用說(shuō)明：
1. 冰浴中徹底融化2×MasterMix，混勻后Minispin將溶液收集到管底。
2. 按照下表在0.2ml PCR管中制備反應(yīng)體系：
|
|
50μl反應(yīng)體系
|
終濃度
|
|
2×MasterMix
|
25μl
|
1×
|
|
上游引物 10μM
|
1-5μl
|
0.2-0.8 μM
|
|
下游引物 10μM
|
1-5μl
|
0.2-0.8 μM
|
|
模板
|
× μl
|
10pg-1μg
|
|
水
|
補(bǔ)至50μl
|
|
3. 快甩離心將反應(yīng)液收集到管底。
4. PCR儀如果沒(méi)有熱蓋加熱的話，補(bǔ)加25μl礦物油。
5. PCR儀上執(zhí)行以下程序：
|
步驟
|
溫度
|
時(shí)間
|
循環(huán)數(shù)
|
|
初始變性
|
94℃
|
5 min
|
1
|
|
變性
|
94℃
|
30 sec
|
25-40*
|
|
退火
|
Tm-5℃*
|
30 sec
|
|
延伸
|
72℃
|
1 min/kb
|
|
最后延伸
|
72℃
|
5 min
|
1
|
*注： PCR 反應(yīng)條件視模板、引物等的結(jié)構(gòu)條件不同而各異。在實(shí)際操作中需根據(jù)模板、目的片段的大小、堿基序列和引物的長(zhǎng)短等具體情況，設(shè)定最佳的反應(yīng)條件（溫度、時(shí)間等）。
產(chǎn)品示例：
2×Taq PCR MasterMix擴(kuò)增效果比較
M III：RTM403 DNA Marker III
lane 1：Rice 5000 bp
lane 2：Ricie 3000 bp
lane 3: Rice 600 bp
引用RTC3104 2×Taq PCR MasterMix含染料發(fā)表文章
1. [2013 IF=1.63] Preventive effect of
total glycosides from Ligustri Lucidi Fructus against nonalcoholic fatty liver
in mice
Author: Nianyun Yang, Yiwen Zhang and Jianming Guo
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Z. Naturforsch. 2015; 70(9-10)c:
237–241
Institution：Nanjing University of Chinese Medicine
Paper link： https://pubmed.ncbi.nlm.nih.gov/26501160/
2. [2015 IF=1.377] Expression and
localization of guanine
nucleotide-binding protein
alpha S in
the testis and
epididymis of rams
at different developmental stages
Author: Zhen Li, Jieli
Lu, Jia Chen,
Quanhai Pang, Ruipeng
Nan, Zhiwei Zhu
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Animal
Reproduction Science. Volume
178, March 2017, Pages 31-39
Institution：Shanxi Agricultural University
Paper link：https://www.sciencedirect.com/science/article/pii/S0378432016307187
3. [2018 IF=2.9] Integration of gene
expression profile data to screen and verify immune-related genes of chicken
erythrocytes involved in disease virus Marek’s
Author: Ali Raza Jahejo, Sayyad Ali Raza Bukhari, Fa-Jie Jia,
Sayed Haidar Abbas Raza, Mujahid Ali Shah, Nasir Rajput, Anam Ahsan, Sheng Niu,
Guan-Bao Ning, Ding Zhang, Yu-Hai Bi, Qi-Hui Wang, Ling-Xia Han, Wen-Xia Tian
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Microbial Pathogenesis. 148 (2020)
104454
Institution：Shanxi Agricultural University
Paper link：https://doi.org/10.1016/j.micpath.2020.104454
4. [2018 IF=1.69] Study on the correlation
between BMPR1Bprotein in sheep blood and reproductive performance
Author: Xiaoyan Zhang, Liping Zhang, Weibo Sun, Xia Lang,
Jianping Wu, Caiye Zhu,Jianlei Jia, Jinpen Jin, Younfu La, David P. Casper
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Journal of Animal Science. 2020 May
1;98(5)
Institution：Gansu Agricultural University
Paper link：https://pubmed.ncbi.nlm.nih.gov/32300800/
5. [2019 IF=2.6] Natural occurrence
of Alternaria mycotoxins in wheat and potential of reducing
associated risks using magnolol.
Author: Dongmei Jiang, DizheWei, Hongtao Li,LiuqingWang,Nan
Jiang,Yunchao Li, Meng Wang
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Journal of the Science
of Food and Agriculture. Volume101, Issue7,May 2021
Institution：Beijing Academy of Agriculture and
Forestry Sciences
Paper link： https://doi.org/10.1002/jsfa.10901
6. [2022 IF=6.9] Galactinol synthase 2
infuences the metabolism of chlorophyll, carotenoids, and ethylene in tomato
fruits.
Author: Huidong Zhang, Kunpeng Zhang, Xueya Zhao, Mengxi Bi,
Yudong Liu, Shuo Wang, Yi He，Kui Ma, Mingfang Qi
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Journal of Experimental Botany Published:
15 March 2024
Institution：College of Horticulture, Shenyang
Agricultural University
Paper link： https://doi.org/10.1093/jxb/erae121
7. [2023 IF=3.3] Construction of Genetic
Linkage Maps Using SSR Markers and Identification of Flower Bud-Related QTLs in
Nightlily (Hemerocallis citrina).
Author: Feifan Hou, Xufei Liang, Rui Chen, Xuan Ji, Hongtao Li,
Mengyue Jing, Yang Gao，Yuting Liu, Sen Li, Guoming Xing，Yanfang Wang
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Agronomy 2025, 15, 534
Institution：College of Horticulture, Shanxi
Agricultural University
Paper link：https://doi.org/10.3390/agronomy15030534